The Bmp gradient of the zebrafish gastrula guides migrating lateral cells by regulating cell-cell adhesion Journal Article


Author(s): von der Hardt, Sophia; Bakkers, Jeroen; Inbal, Adi; Carvalho, Lara; Solnica-Krezel, Lilianna; Heisenberg, Carl-Philipp; Hammerschmidt, Matthias
Article Title: The Bmp gradient of the zebrafish gastrula guides migrating lateral cells by regulating cell-cell adhesion
Affiliation
Abstract: Background: Bone morphogenetic proteins (Bmps) are required for the specification of ventrolateral cell fates during embryonic dorsoventral patterning and for proper convergence and extension gastrulation movements, but the mechanisms underlying the latter role remained elusive. Results: Via bead implantations, we show that the Bmp gradient determines the direction of lateral mesodermal cell migration during dorsal convergence in the zebrafish gastrula. This effect is independent of its role during dorsoventral patterning and of noncanonical Wnt signaling. However, it requires Bmp signal transduction through AIk8 and Smad5 to negatively regulate Ca2+/Cadherin-dependent cell-cell adhesiveness. In vivo, converging mesodermal cells form lamellipodia that attach to adjacent cells. Bmp signaling diminishes the Cadherin-dependent stability of such contact points, thereby abrogating subsequent cell displacement during lamellipodial retraction. Conclusions: We propose that the ventral-to-dorsal Bmp gradient has an instructive role to establish a reverse gradient of cell-cell adhesiveness, thereby defining different migratory zones and directing lamellipodia-driven cell migrations during dorsal convergence in lateral regions of the zebrafish gastrula.
Keywords: DEVBIO
Journal Title: Current Biology
Volume: 17
Issue 6
ISSN: 0960-9822
Publisher: Cell Press  
Date Published: 2007-03-20
Start Page: 475
End Page: 487
DOI: 10.1016/j.cub.2007.02.013
Open access: no