A small number of open Ca(2+) channels trigger transmitter release at a central GABAergic synapse Journal Article


Author(s): Bucurenciu, Iancu; Bischofberger, Josef; Jonas, Peter
Article Title: A small number of open Ca(2+) channels trigger transmitter release at a central GABAergic synapse
Affiliation
Abstract: To determine the number of open Ca(2+) channels necessary for transmitter release at the inhibitory basket cell-granule cell synapse in rat hippocampus, we combined presynaptic Ca(2+) imaging, recording of postsynaptic currents and modeling. We found that that the opening of three or fewer Ca(2+) channels triggered transmitter release. Furthermore, a small number of Ca(2+) channels were able to evoke release with high temporal precision, despite stochastic Ca(2+) channel opening.
Keywords: Animals; Rats; Stochastic Processes; Calcium/metabolism; Animals, Newborn; Calcium Channel Blockers/pharmacology; Chelating Agents/pharmacology; Egtazic Acid/analogs & derivatives/pharmacology; Exocytosis/drug effects/physiology; Hippocampus/cytology; Inhibitory Postsynaptic Potentials/drug effects/physiology; Ion Channel Gating/drug effects/physiology; Models, Neurological; Patch-Clamp Techniques; Rats, Wistar; Synapses/drug effects/physiology; omega-Agatoxin IVA/pharmacology; Calcium Channels/physiology; Calcium Channels, N-Type/physiology; gamma-Aminobutyric Acid/metabolism; Neurotransmitter Agents/metabolism; Neurons/cytology; Presynaptic Terminals/drug effects/physiology
Journal Title: Nature Neuroscience
Volume: 13
Issue 1
ISSN: 1546-1726
Publisher: Nature Publishing Group  
Publication Place: United States
Date Published: 2010-01-01
Start Page: 19
End Page: 21
DOI: 10.1038/nn.2461
Open access: no