Combinatorial synthesis of genetic networks Journal Article


Author(s): Guet, Cǎlin C; Elowitz, Michael B.; Hsing, Weihong; Leibler, Stanislas
Article Title: Combinatorial synthesis of genetic networks
Affiliation
Abstract: A central problem in biology is determining how genes interact as parts of functional networks. Creation and analysis of synthetic networks, composed of well-characterized genetic elements, provide a framework for theoretical modeling. Here, with the use of a combinatorial method, a library of networks with varying connectivity was generated in Escherichia coli. These networks were composed of genes encoding the transcriptional regulators Lacl, TetR, and lambda Cl, as well as the corresponding promoters. They displayed phenotypic behaviors resembling binary logical circuits, with two chemical “inputs” and a fluorescent protein “output.” Within this simple system, diverse computational functions arose through changes in network connectivity. Combinatorial synthesis provides an alternative approach for studying biological networks, as well as an efficient method for producing diverse phenotypes in vivo.
Journal Title: Science
Volume: 296
Issue 5572
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science  
Date Published: 2002-01-01
Start Page: 1466
End Page: 1470
URL:
DOI: 10.1126/science.1067407
Open access: no
IST Austria Authors
  1. Călin Guet
    31 Guet
Related IST Austria Work