Protein expression enhancement in efflux-deleted mutant bacteria Journal Article


Author(s): Le, Thuc T.; Guet, Cǎlin C; Cluzel, Philippe
Article Title: Protein expression enhancement in efflux-deleted mutant bacteria
Affiliation
Abstract: We applied a single-cell assay to characterize how transcription dynamics affects protein expression levels of a tetracycline-inducible gene expression system. Transcriptional activity of the tetracycline promoter in response to a steady level of inducer is steady in ΔacrAB efflux mutant but pulsating in wildtype Escherichia coli cells. We found that the expression level of the green fluorescent protein is several folds higher in ΔacrAB efflux mutant than in wildtype cells.
Keywords: Time Factors; Recombinant Proteins; Green Fluorescent Proteins/metabolism; Green Fluorescent Proteins; Green Fluorescent Proteins/genetics; Gene Expression Regulation, Bacterial; Escherichia coli/genetics; Efflux pump; Single cell; GFP; Inducible vector; Escherichia coli/metabolism; Gene Deletion; Promoter Regions, Genetic; Protein Engineering; Recombinant Proteins/biosynthesis; Recombinant Proteins/genetics; Tetracycline Resistance
Journal Title: Protein Expression and Purification
Volume: 48
Issue 1
ISSN: 1046-5928
Publisher: Elsevier  
Date Published: 2006-07-01
Start Page: 28
End Page: 31
Open access: no