Corelease of two fast neurotransmitters at a central synapse Journal Article


Author(s): Jonas, Peter; Bischofberger, Joseph; Sandkühler, Jürgen
Article Title: Corelease of two fast neurotransmitters at a central synapse
Affiliation
Abstract: It is widely accepted that individual neurons in the central nervous system release only a single fast transmitter. The possibility of corelease of fast neurotransmitters was examined by making paired recordings from synaptically connected neurons in spinal cord slices. Unitary inhibitory postsynaptic currents generated at interneuron-motoneuron synapses consisted of a strychnine-sensitive, glycine receptor-mediated component and a bicuculline-sensitive, γ-aminobutyric acid (GABA)(A) receptor-mediated component. These results indicate that spinal interneurons release both glycine and GABA to activate functionally distinct receptors in their postsynaptic target cells. A subset of miniature synaptic currents also showed both components, consistent with corelease from individual synaptic vesicles.
Keywords: Animals; Rats; Synaptic Transmission; Patch-Clamp Techniques; Rats, Wistar; Spinal cord; Interneurons; gamma-Aminobutyric Acid; Receptors, GABA-A; GABA Antagonists; Presynaptic Terminals; Baclofen; Bicuculline; Glycine; Glycine Agents; Motor Neurons; Receptors, GABA-B; Receptors, Glycine; Strychnine; Synaptic Vesicles
Journal Title: Science
Volume: 281
Issue 5375
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science  
Date Published: 1998-07-17
Start Page: 419
End Page: 424
DOI: 10.1126/science.281.5375.419
Notes: see comment by Nicoll RA, Malenka RC (1998) Science 281:360-361
Open access: no