Relative abundance of subunit mRNAs determines gating and Ca(2+) permeability of AMPA receptors in principal neurons and interneurons in rat CNS Journal Article


Author(s): Geiger, Jörg R; Melcher, Thorsten; Koh, Duk S; Sakmann, Bert; Seeburg, Peter H; Jonas, Peter; Monyer, Hannah
Article Title: Relative abundance of subunit mRNAs determines gating and Ca(2+) permeability of AMPA receptors in principal neurons and interneurons in rat CNS
Affiliation
Abstract: Recording of glutamate-activated currents in membrane patches was combine with RT-PCR-mediated AMPA receptor (AMPAR) subunit mRNA analysis in single identified cells of rat brain slices. Analysis of AMPARs in principal neurons end interneurons of hippocampus and neocortex and in auditory relay neurons and Bergmann glial cells indicates that the GluR-B subunit in its flip version determines formation of receptors with relatively slow gating, whereas the GluR-D subunit promotes assembly of more rapidly gated receptors. The relation between Ca 2+ permeability of AMPAR channels and the relative GluR-B mRNA abundance is consistent with the dominance of this subunit in determining the Ca 2+ permeability of native receptors. The results suggest that differential expression of GluR-B and GluR-D subunit genes, as well as splicing end editing of their mRNAs, account for the differences in gating and Ca 2+ permeability of native AMPAR channels.
Keywords: Rats; Molecular Sequence Data; Base Sequence; Polymerase Chain Reaction; Calcium; Patch-Clamp Techniques; Rats, Wistar; Ion Channel Gating; Neurons; RNA, Messenger; animal; Cell Membrane Permeability; Receptors, AMPA; Auditory Pathways; Central Nervous System; Cerebellum; Hippocampus; Interneurons; Neuroglia; Pyramidal Cells; Support, Non-U.S. Gov't
Journal Title: Neuron
Volume: 15
Issue 1
ISSN: 0896-6273
Publisher: Elsevier  
Date Published: 1995-07-01
Start Page: 193
End Page: 204
Copyright Statement: © Copyright 2007 Elsevier B.V., All rights reserved.
DOI: 10.1016/0896-6273(95)90076-4
Open access: no