Modern ionotropic glutamate receptor with a K+ selectivity signature sequence Journal Article


Author(s): Janovjak, Harald; Sandoz, Guillaume; Isacoff, Ehud Y
Article Title: Modern ionotropic glutamate receptor with a K+ selectivity signature sequence
Affiliation IST Austria
Abstract: Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system and gates non-selective cation channels. The origins of glutamate receptors are not well understood as they differ structurally and functionally from simple bacterial ligand-gated ion channels. Here we report the discovery of an ionotropic glutamate receptor that combines the typical eukaryotic domain architecture with the 'TXVGYG' signature sequence of the selectivity filter found in K+ channels. This receptor exhibits functional properties intermediate between bacterial and eukaryotic glutamate-gated ion channels, suggesting a link in the evolution of ionotropic glutamate receptors.
Keywords: Animals; Amino Acid Sequence; Molecular Sequence Data; Phylogeny; Sequence Alignment; Gene Expression; Recombinant Proteins; Potassium; Potassium/metabolism; Glutamic Acid/metabolism; Ion Channel Gating/physiology; RNA, Messenger/metabolism; RNA, Messenger; Recombinant Proteins/genetics; ; Xenopus laevis; Glutamic Acid; Recombinant Proteins/metabolism; Amino Acid Motifs; Bacteria; Databases, Genetic; Oocytes/cytology; Oocytes/physiology; Plants; RNA, Messenger/genetics; Receptors, Ionotropic Glutamate/genetics; Receptors, Ionotropic Glutamate/metabolism; Rotifera/chemistry; Rotifera/genetics; Rotifera/metabolism; Transfection; Receptors, Ionotropic Glutamate
Journal Title: Nature Communications
Volume: 2
Issue 232
ISSN: 2041-1723
Publisher: Nature Publishing Group  
Date Published: 2011-03-08
Start Page: 1
End Page: 6
Sponsor: PN2EY018241/EY/NEI NIH HHS/United States; R01 NS035549-14/NS/NINDS NIH HHS/United States; R01 NS35549/NS/NINDS NIH HHS/United States
URL:
DOI: 10.1038/ncomms1231
Notes: We thank E. Gladyshev and I. Arkhipova for A. vaga genomic DNA and P. Burkhardt, L. Chen and N. King for helpful discussions; S. Nabavi Nouri for technical assistance; A. Reiner for his gift of the GluK2 expression vector and S.A. Nichols and K. Sjölander for advice on phylogenetic analysis.
Open access: yes (repository)