Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo Journal Article

Author(s): Schachtner, Hannah; Li, Ang; Stevenson, David; Calaminus, Simon D; Thomas, Steven G; Watson, Steve P; Sixt, Michael; Wedlich-Söldner, Roland; Strathdee, Douglas; Machesky, Laura M
Article Title: Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo
Affiliation IST Austria
Abstract: We describe here the development and characterization of a conditionally inducible mouse model expressing Lifeact-GFP, a peptide that reports the dynamics of filamentous actin. We have used this model to study platelets, megakaryocytes and melanoblasts and we provide evidence that Lifeact-GFP is a useful reporter in these cell types ex vivo. In the case of platelets and megakaryocytes, these cells are not transfectable by traditional methods, so conditional activation of Lifeact allows the study of actin dynamics in these cells live. We studied melanoblasts in native skin explants from embryos, allowing the visualization of live actin dynamics during cytokinesis and migration. Our study revealed that melanoblasts lacking the small GTPase Rac1 show a delay in the formation of new pseudopodia following cytokinesis that accounts for the previously reported cytokinesis delay in these cells. Thus, through use of this mouse model, we were able to gain insights into the actin dynamics of cells that could only previously be studied using fixed specimens or following isolation from their native tissue environment.
Keywords: cell migration; Cytokinesis; Actin dynamics; Cytoskeleton; Imaging No items selected; Motility
Journal Title: European Journal of Cell Biology
Volume: 91
Issue 11-12
ISSN: 0070-2463
Publisher: Elsevier  
Date Published: 2012-11-01
Start Page: 923
End Page: 929
DOI: 10.1016/j.ejcb.2012.04.002
Open access: yes (repository)