Peroxiredoxin stabilization of DE-cadherin promotes primordial germ cell adhesion Journal Article


Author(s): DeGennaro, Matthew; Hurd, Thomas R; Siekhaus, Daria; Biteau, Benoit; Jasper, Heinrich; Lehmann, Ruth
Article Title: Peroxiredoxin stabilization of DE-cadherin promotes primordial germ cell adhesion
Affiliation
Abstract: Regulated adhesion between cells and their environment is critical for normal cell migration. We have identified mutations in a gene encoding the Drosophila hydrogen peroxide (H2O2)-degrading enzyme Jafrac1, which lead to germ cell adhesion defects. During gastrulation, primordial germ cells (PGCs) associate tightly with the invaginating midgut primordium as it enters the embryo; however, in embryos from jafrac1 mutant mothers this association is disrupted, leaving some PGCs trailing on the outside of the embryo. We observed similar phenotypes in embryos from DE-cadherin/shotgun (shg) mutant mothers and were able to rescue the jafrac1 phenotype by increasing DE-cadherin levels. This and our biochemical evidence strongly suggest that Jafrac1-mediated reduction of H2O2 is required to maintain DE-cadherin protein levels in the early embryo. Our results present in vivo evidence of a peroxiredoxin regulating DE-cadherin-mediated adhesion.
Keywords: Animals; Transcription, Genetic; Cell Adhesion; Endocytosis; Gene Expression Regulation; Drosophila Proteins; Gastrulation; protein stability; cell aggregation; Drosophila melanogaster; Embryo, Nonmammalian; Cadherins; Germ Cells; Adherens Junctions; Cell Survival; Hydrogen Peroxide; Oxidative Stress; Peroxidases
Journal Title: Developmental Cell
Volume: 20
Issue 2
ISSN: 1534-5807
Publisher: Cell Press  
Date Published: 2011-02-15
Start Page: 233
End Page: 243
DOI: 10.1016/j.devcel.2010.12.007
Open access: no