ABP1 and ROP6 GTPase signaling regulate clathrin mediated endocytosis in Arabidopsis roots Journal Article


Author(s): Chen, Xu; Naramoto, Satoshi; Robert, Stéphanie; Tejos, Ricardo; Löfke, Christian; Lin, Deshu; Yang, Zhenbiao; Friml, Jiří
Article Title: ABP1 and ROP6 GTPase signaling regulate clathrin mediated endocytosis in Arabidopsis roots
Affiliation
Abstract: The dynamic spatial and temporal distribution of the crucial plant signaling molecule auxin is achieved by feedback coordination of auxin signaling and intercellular auxin transport pathways [1, 2]. Developmental roles of auxin have been attributed predominantly to its effect on transcription; however, an alternative pathway involving AUXIN BINDING PROTEIN1 (ABP1) has been proposed to regulate clathrin-mediated endocytosis in roots and Rho-like GTPase (ROP)-dependent pavement cell interdigitation in leaves [3, 4]. In this study, we show that ROP6 and its downstream effector RIC1 regulate clathrin association with the plasma membrane for clathrin-mediated endocytosis, as well as for its feedback regulation by auxin. Genetic analysis revealed that ROP6/RIC1 acts downstream of ABP1 to regulate endocytosis. This signaling circuit is also involved in the feedback regulation of PIN-FORMED 1 (PIN1) and PIN2 auxin transporters activity (via its constitutive endocytosis) and corresponding auxin transport-mediated processes, including root gravitropism and leave vascular tissue patterning. Our findings suggest that the signaling module auxin-ABP1-ROP6/RIC1-clathrin-PIN1/PIN2 is a shared component of the feedback regulation of auxin transport during both root and aerial development.
Keywords: Arabidopsis
Journal Title: Current Biology
Volume: 22
Issue 14
ISSN: 0960-9822
Publisher: Cell Press  
Date Published: 2012-07-24
Start Page: 1326
End Page: 1332
DOI: 10.1016/j.cub.2012.05.020
Open access: no