Cell plate restricted association of DRP1A and PIN proteins is required for cell polarity establishment in arabidopsis Journal Article


Author(s): Mravec, Jozef; Petrášek, Jan; Li, Na; Boeren, Sjef; Karlova, Rumyana; Kitakura, Saeko; Pařezová, Markéta; Naramoto, Satoshi; Nodzyński, Thomasz; Dhonukshe, Pankaj; Bednarek, Sebastian Y; Zažímalová, Eva; De Vries, Sacco; Friml, Jiří
Article Title: Cell plate restricted association of DRP1A and PIN proteins is required for cell polarity establishment in arabidopsis
Affiliation
Abstract: The polarized transport of the phytohormone auxin [1], which is crucial for the regulation of different stages of plant development [2, 3], depends on the asymmetric plasma membrane distribution of the PIN-FORMED (PIN) auxin efflux carriers [4, 5]. The PIN polar localization results from clathrin-mediated endocytosis (CME) from the plasma membrane and subsequent polar recycling [6]. The Arabidopsis genome encodes two groups of dynamin-related proteins (DRPs) that show homology to mammalian dynamin - a protein required for fission of endocytic vesicles during CME [7, 8]. Here we show by coimmunoprecipitation (coIP), bimolecular fluorescence complementation (BiFC), and Förster resonance energy transfer (FRET) that members of the DRP1 group closely associate with PIN proteins at the cell plate. Localization and phenotypic analysis of novel drp1 mutants revealed a requirement for DRP1 function in correct PIN distribution and in auxin-mediated development. We propose that rapid and specific internalization of PIN proteins mediated by the DRP1 proteins and the associated CME machinery from the cell plate membranes during cytokinesis is an important mechanism for proper polar PIN positioning in interphase cells.
Keywords: Amino Acid Sequence; Molecular Sequence Data; Green Fluorescent Proteins; Cell polarity; Arabidopsis; Arabidopsis Proteins; Membrane Transport Proteins; Dynamins; Genome, Plant; Immunoprecipitation; Spectrometry, Fluorescence
Journal Title: Current Biology
Volume: 21
Issue 12
ISSN: 0960-9822
Publisher: Cell Press  
Date Published: 2011-06-21
Start Page: 1055
End Page: 1060
DOI: 10.1016/j.cub.2011.05.018
Open access: no