Inositol trisphosphate-induced ca^2+ signaling modulates auxin transport and pin polarity Journal Article


Author(s): Zhang, Jing; Vanneste, Steffen; Brewer, Philip B; Michniewicz, Marta; Grones, Peter; Kleine-Vehn, Jürgen; Löfke, Christian; Teichmann, Thomas; Bielach, Agnieszka; Cannoot, Bernard; Hoyerová, Klára; Chen, Xu; Xue, Hong-Wei; Benková, Eva; Zažímalová, Eva; Friml, Jiří
Article Title: Inositol trisphosphate-induced ca^2+ signaling modulates auxin transport and pin polarity
Affiliation
Abstract: The phytohormone auxin is an important determinant of plant development. Directional auxin flow within tissues depends on polar localization of PIN auxin transporters. To explore regulation of PIN-mediated auxin transport, we screened for suppressors of PIN1 overexpression (supo) and identified an inositol polyphosphate 1-phosphatase mutant (supo1), with elevated inositol trisphosphate (InsP 3) and cytosolic Ca 2+ levels. Pharmacological and genetic increases in InsP 3 or Ca 2+ levels also suppressed the PIN1 gain-of-function phenotypes and caused defects in basal PIN localization, auxin transport and auxin-mediated development. In contrast, the reductions in InsP 3 levels and Ca 2+ signaling antagonized the effects of the supo1 mutation and disrupted preferentially apical PIN localization. InsP 3 and Ca 2+ are evolutionarily conserved second messengers involved in various cellular functions, particularly stress responses. Our findings implicate them as modifiers of cell polarity and polar auxin transport, and highlight a potential integration point through which Ca 2+ signaling-related stimuli could influence auxin-mediated development.
Keywords: Calcium; Blotting, Western; Signal Transduction; Gene Expression Regulation, Plant; Cell polarity; Arabidopsis; Arabidopsis Proteins; Indoleacetic Acids; Membrane Transport Proteins; Mutant Proteins; Cytosol; Inositol 1,4,5-Trisphosphate; Phosphoric Monoester Hydrolases
Journal Title: Developmental Cell
Volume: 20
Issue 6
ISSN: 1534-5807
Publisher: Cell Press  
Date Published: 2011-06-14
Start Page: 855
End Page: 866
DOI: 10.1016/j.devcel.2011.05.013
Open access: no