ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis Journal Article


Author(s): Robert, Stéphanie; Kleine-Vehn, Jürgen; Barbez, Elke; Sauer, Michael; Paciorek, Tomasz; Baster, Paweł; Vanneste, Steffen; Zhang, Jing; Simon, Sibu; Čovanová, Milada; Hayashi, Kenichiro; Dhonukshe, Pankaj; Yang, Zhenbiao; Bednarek, Sebastian Y; Jones, Alan M; Luschnig, Christian; Aniento, Fernando; Zažímalová, Eva; Friml, Jiří
Article Title: ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis
Affiliation
Abstract: Spatial distribution of the plant hormone auxin regulates multiple aspects of plant development. These self-regulating auxin gradients are established by the action of PIN auxin transporters, whose activity is regulated by their constitutive cycling between the plasma membrane and endosomes. Here, we show that auxin signaling by the auxin receptor AUXIN-BINDING PROTEIN 1 (ABP1) inhibits the clathrin-mediated internalization of PIN proteins. ABP1 acts as a positive factor in clathrin recruitment to the plasma membrane, thereby promoting endocytosis. Auxin binding to ABP1 interferes with this action and leads to the inhibition of clathrin-mediated endocytosis. Our study demonstrates that ABP1 mediates a nontranscriptional auxin signaling that regulates the evolutionarily conserved process of clathrin-mediated endocytosis and suggests that this signaling may be essential for the developmentally important feedback of auxin on its own transport.
Keywords: Endocytosis; Receptors, Cell Surface; Arabidopsis; Arabidopsis Proteins; Indoleacetic Acids; Cell Membrane; Membrane Transport Proteins; Plant Proteins; Clathrin
Journal Title: Cell
Volume: 143
Issue 1
ISSN: 0092-8674
Publisher: Cell Press  
Date Published: 2010-10-01
Start Page: 111
End Page: 121
DOI: 10.1016/j.cell.2010.09.027
Open access: no