Interactions of PIN and PGP auxin transport mechanisms Conference Paper


Author(s): Bandyopadhyay, Anindita; Blakeslee, Joshua; Lee, Ok Ran; Mravec, Jozef; Sauer, Michael; Titapiwatanakun, Boosaree; Makam, Srinivas N; Bouchard, Rodolphe; Geisler, Markus; Martinoia, Enrico; Friml, Jiří; Peer, Wendy A; Murphy, Angus S
Title: Interactions of PIN and PGP auxin transport mechanisms
Title Series: Biochemical Society Transactions
Affiliation
Abstract: Polarized transport of the plant hormone auxin influences multiple growth processes in plants and is regulated by plasma-membrane-localized efflux and uptake carriers. The PGP (P-glycoprotein) ABC transporters (ATP-binding-cassette transporters), PIN (pin-formed) subfamily of major facilitator proteins and members of AUX/LAX families have been shown to independently transport auxin both in planta and in heterologous systems. However, PIN- and PGP-mediated transport in heterologous systems exhibits decreased substrate specificity and inhibitor-sensitivity compared with what is seen in plants and plant cells. To determine whether PIN-PGP interactions enhance transport specificity, we analysed interactions of the representative auxin-transporting PGPs with PIN1 and AUX1 in planta and in heterologous systems. Here, we provide evidence that PINs and PGPs interact and function both independently and co-ordinately to control polar auxin transport and impart transport specificity and directionality. These interactions take place in protein complexes stabilized by PGPs in detergent-resistant microdomains.
Keywords: Auxin; AUX1; Detergent-resistant microdomain (DRM); P-glycoprotein (PGP); Pin-formed (PIN); Polar transport
Conference Title: Intercellular Signalling in Plants
Volume: 35
Issue 1
Conference Dates: September 4-5, 2006
Conference Location: Sheffield, U.K.
ISBN: 1470-8752
Publisher: Portland Press  
Date Published: 2007-02-01
Start Page: 137
End Page: 141
DOI: 10.1042/BST0350137
Open access: no