PIN proteins perform a rate-limiting function in cellular auxin efflux Journal Article


Author(s): Petrášek, Jan; Mravec, Jozef; Bouchard, Rodolphe; Blakeslee, Joshua; Abas, Melinda; Seifertová, Daniela; Wiśniewska, Justyna; Tadele, Zerihun; Kubeš, Martin; Čovanová, Milada; Dhonukshe, Pankaj; Skůpa, Petr; Benková, Eva; Perry, Lucie; Křeček, Pavel; Lee, Ok Ran; Fink, Gerald R; Geisler, Markus; Murphy, Angus S; Luschnig, Christian; Zažímalová, Eva; Friml, Jiří
Article Title: PIN proteins perform a rate-limiting function in cellular auxin efflux
Affiliation
Abstract: Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
Keywords: Humans; Cells, Cultured; Mutation; Kinetics; Biological Transport; plant roots; Saccharomyces cerevisiae; Transfection; Gravitropism; Arabidopsis; Arabidopsis Proteins; Indoleacetic Acids; Cell Membrane; Membrane Transport Proteins; Tobacco; ATP-Binding Cassette Transporters; Phthalimides; Hela Cells; Naphthaleneacetic Acids; Transformation, Genetic
Journal Title: Science
Volume: 312
Issue 5775
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science  
Date Published: 2006-05-12
Start Page: 914
End Page: 918
DOI: 10.1126/science.1123542
Open access: no