Endocytosis of cell surface material mediates cell plate formation during plant cytokinesis Journal Article


Author(s): Dhonukshe, Pankaj; Baluška, František; Schlicht, Markus; Hlavacka, Andrej; Šamaj, Jozef; Friml, Jiří; Gadella, Theodorus W
Article Title: Endocytosis of cell surface material mediates cell plate formation during plant cytokinesis
Affiliation
Abstract: Dividing plant cells perform a remarkable task of building a new cell wall within the cytoplasm in a few minutes. A long-standing paradigm claims that this primordial cell wall, known as the cell plate, is generated by delivery of newly synthesized material from Golgi apparatus-originated secretory vesicles. Here, we show that, in diverse plant species, cell surface material, including plasma membrane proteins, cell wall components, and exogenously applied endocytic tracers, is rapidly delivered to the forming cell plate. Importantly, this occurs even when de novo protein synthesis is blocked. In addition, cytokinesis-specific syntaxin KNOLLE as well as plasma membrane (PM) resident proteins localize to endosomes that fuse to initiate the cell plate. The rate of endocytosis is strongly enhanced during cell plate formation, and its genetic or pharmacological inhibition leads to cytokinesis defects. Our results reveal that endocytic delivery of cell surface material significantly contributes to cell plate formation during plant cytokinesis.
Keywords: Models, Biological; Time Factors; Qa-SNARE Proteins; Fluorescent Antibody Technique; Blotting, Western; Microscopy, Confocal; Endocytosis; Gene Expression Regulation, Plant; Cytoplasm; Plants; Membrane Proteins; Cytokinesis; endosomes; Microscopy, Immunoelectron; Gene Transfer Techniques; Arabidopsis Proteins; Guanine Nucleotide Exchange Factors; Cell Membrane; Membrane Transport Proteins; Plant Proteins; rab GTP-Binding Proteins; Brefeldin A; Cycloheximide; Pectins; Pinocytosis; Protein Synthesis Inhibitors; Pyridinium Compounds; Quaternary Ammonium Compounds; Vesicular Transport Proteins
Journal Title: Developmental Cell
Volume: 10
Issue 1
ISSN: 1534-5807
Publisher: Cell Press  
Date Published: 2006-01-01
Start Page: 137
End Page: 150
DOI: 10.1016/j.devcel.2005.11.015
Open access: no