A PINOID-dependent binary switch in apical-basal PIN polar targeting directs auxin efflux Journal Article


Author(s): Friml, Jiří; Yang, Xiong; Michniewicz, Marta; Weijers, Dolf; Quint, Ab; Tietz, Olaf; Benjamins, René; Ouwerkerk, Pieter B; Ljung, Karin; Sandberg, Göran; Hooykaas, Paul J; Palme, Klaus; Offringa, Remko
Article Title: A PINOID-dependent binary switch in apical-basal PIN polar targeting directs auxin efflux
Affiliation
Abstract: Polar transport-dependent local accumulation of auxin provides positional cues for multiple plant patterning processes. This directional auxin flow depends on the polar subcellular localization of the PIN auxin efflux regulators. Overexpression of the PINOID protein kinase induces a basal-to-apical shift in PIN localization, resulting in the loss of auxin gradients and strong defects in embryo and seedling roots. Conversely, pid loss of function induces an apical-to-basal shift in PIN1 polar targeting at the inflorescence apex, accompanied by defective organogenesis. Our results show that a PINOID-dependent binary switch controls PIN polarity and mediates changes in auxin flow to create local gradients for patterning processes.
Keywords: Mutation; Biological Transport; plant roots; Gene Expression Regulation, Plant; Recombinant Fusion Proteins; Arabidopsis; Arabidopsis Proteins; Indoleacetic Acids; Membrane Transport Proteins; Plants, Genetically Modified; Protein-Serine-Threonine Kinases; Meristem; Seeds; Plant Epidermis; Plant Shoots
Journal Title: Science
Volume: 306
Issue 5697
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science  
Date Published: 2004-10-29
Start Page: 862
End Page: 865
DOI: 10.1126/science.1100618
Open access: no