Investigating the principles of morphogen gradient formation: from tissues to cells Journal Article


Author(s): Kicheva, Anna; Bollenbach, Tobias; Wartlick, Ortrud; Julicher, Frank; Gonzalez-Gaitan, Marcos
Article Title: Investigating the principles of morphogen gradient formation: from tissues to cells
Affiliation IST Austria
Abstract: Morphogen gradients regulate the patterning and growth of many tissues, hence a key question is how they are established and maintained during development. Theoretical descriptions have helped to explain how gradient shape is controlled by the rates of morphogen production, spreading and degradation. These effective rates have been measured using fluorescence recovery after photobleaching (FRAP) and photoactivation. To unravel which molecular events determine the effective rates, such tissue-level assays have been combined with genetic analysis, high-resolution assays, and models that take into account interactions with receptors, extracellular components and trafficking. Nevertheless, because of the natural and experimental data variability, and the underlying assumptions of transport models, it remains challenging to conclusively distinguish between cellular mechanisms.
Journal Title: Current Opinion in Genetics & Development
Volume: 22
Issue 6
ISSN: 0959-437X
Publisher: Elsevier  
Date Published: 2012-12-01
Start Page: 527
End Page: 532
Sponsor: AK is currently supported by an MRC CDF. MGG and OW were supported by the Swiss National Science Foundation, grants from the Swiss SystemsX.ch initiative, LipidX-2008/011, an ERC advanced investigator grant and the Polish-Swiss research program.
DOI: 10.1016/j.gde.2012.08.004
Open access: no