The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS-mediated embryo patterning Journal Article


Author(s): Hamann, Thorsten; Benková, Eva; Bäurle, Isabel; Kientz, Marika; Jürgens, Gerd
Article Title: The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS-mediated embryo patterning
Affiliation
Abstract: Developmental responses to the plant hormone auxin are thought to be mediated by interacting pairs from two protein families: short-lived inhibitory IAA proteins and ARF transcription factors binding to auxin-response elements. Monopteros mutants lacking activating ARF5 and the auxin-insensitive mutant bodenlos fail to initiate the root meristem during early embryogenesis. Here we show that the bodenlos phenotype results from an amino-acid exchange in the conserved degradation domain of IAA12. BODENLOS and MONOPTEROS interact in the yeast two-hybrid assay and the two genes are coexpressed in early embryogenesis, suggesting that BODENLOS inhibits MONOPTEROS action in root meristem initiation.
Keywords: Base Sequence; DNA Primers; Phenotype; Genes, Plant; Gene Expression Regulation; Transcription Factors; DNA-Binding Proteins; Arabidopsis; Arabidopsis Proteins; Seeds; Promoter Regions (Genetics)
Journal Title: Genes and Development
Volume: 16
Issue 13
ISSN: 1549-5477
Publisher: Cold Spring Harbor Laboratory Press  
Date Published: 2002-07-01
Start Page: 1610
End Page: 1615
DOI: 10.1101/gad.229402
Open access: no