Optical control of ligand-gated ion channels Journal Article


Author(s): Szobota, Stephanie; McKenzie, Catherine; Janovjak, Harald
Article Title: Optical control of ligand-gated ion channels
Affiliation IST Austria
Abstract: In the vibrant field of optogenetics, optics and genetic targeting are combined to commandeer cellular functions, such as the neuronal action potential, by optically stimulating light-sensitive ion channels expressed in the cell membrane. One broadly applicable manifestation of this approach are covalently attached photochromic tethered ligands (PTLs) that allow activating ligand-gated ion channels with outstanding spatial and temporal resolution. Here, we describe all steps towards the successful development and application of PTL-gated ion channels in cell lines and primary cells. The basis for these experiments forms a combination of molecular modeling, genetic engineering, cell culture, and electrophysiology. The light-gated glutamate receptor (LiGluR), which consists of the PTL-functionalized GluK2 receptor, serves as a model.
Keywords: Optical switch; Azobenzene; Glutamate receptor; Optogenetics; Optochemical genetics; Photochromic tethered ligand; LiGluR
Journal Title: Methods in Molecular Biology
Volume: 998
ISSN: 1064-3745
Publisher: Springer  
Date Published: 2013-06-03
Start Page: 417
End Page: 435
Sponsor: Our research is supported by grants of the European Union Seventh Framework Programme (to H.J.) and the Human Frontier Science Program (to H.J.).
URL:
DOI: 10.1007/978-1-62703-351-0_32
Open access: yes (repository)