The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain Journal Article


Author(s): Landberg, Katarina; Pederson, Eric R; Viaene, Tom; Bozorg, Behruz; Friml, Jiří; Jönsson, Henrik; Thelander, Mattias; Sundberg, Eva
Article Title: The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain
Affiliation IST Austria
Abstract: In order to establish a reference for analysis of the function of auxin and the auxin biosynthesis regulators SHORT INTERNODE/ STYLISH (SHI/STY) during Physcomitrella patens reproductive development, we have described male (antheridial) and female (archegonial) development in detail, including temporal and positional information of organ initiation. This has allowed us to define discrete stages of organ morphogenesis and to show that reproductive organ development in P. patens is highly organized and that organ phyllotaxis differs between vegetative and reproductive development. Using the PpSHI1 and PpSHI2 reporter and knockout lines, the auxin reporters GmGH3pro:GUS and PpPINApro:GFP-GUS, and the auxin-conjugating transgene PpSHI2pro:IAAL, we could show that the PpSHI genes, and by inference also auxin, play important roles for reproductive organ development in moss. The PpSHI genes are required for the apical opening of the reproductive organs, the final differentiation of the egg cell, and the progression of canal cells into a cell death program. The apical cells of the archegonium, the canal cells, and the egg cell are also sites of auxin responsiveness and are affected by reduced levels of active auxin, suggesting that auxin mediates PpSHI function in the reproductive organs.
Journal Title: Plant Physiology
Volume: 162
Issue 3
ISSN: 1532-2548
Publisher: American Society of Plant Biologists  
Date Published: 2013-07-01
Start Page: 1406
End Page: 1419
Sponsor: The Swedish Research Council (ES, MT and HJ), the Carl Tryggers Foundation (MT), the Nilsson-Ehle Foundation (KL), and the Gatsby Charitable Foundation (HJ).
URL:
DOI: 10.1104/pp.113.214023
Notes: We thank Isabel Valsecchi and Chen Song for technical assistance, Jian Xu for the pG2NBL-IAAH-NOST plasmid, Lars Ostergaard for the pCR2.1-IAAL plasmid, Mitsuyasu Hasebe for the pCMAK1 plasmid, and Magnus Eklund, Tom Martin, and two anonymous reviewers for valuable comments on the manuscript.
Open access: yes (repository)