Bioimaging with two-photon-induced luminescence from triangular nanoplates and nanoparticle aggregates of gold Journal Article


Author(s): Jiang, Yuqiang; Horimoto, Noriko N; Imura, Kohei; Matsui, Ko; Shigemoto, Ryuichi
Article Title: Bioimaging with two-photon-induced luminescence from triangular nanoplates and nanoparticle aggregates of gold
Affiliation
Abstract: The living cell imaging using a two-photon microscope using gold nanoplates and nanoparticle aggregates was demonstrated. The dimensions of the nanoplates were determined through scanning electron microscopy (SEM) and atomic force microscopy. The height of a 100 nm base-length nanotriangle was around 10 nm, while the height of 300 nm base-length nanotriangle was around 12 nm. A spectrophotometer was also used to determine the extinction spectra of gold nanoparticle colloids. Two-photon-induced photoluminescence (TPIPL) under far-field excitation was tested for gold nanoplates on a glass substrate using two-photon laser scanning microscopy (TPLSM). It was observed that living-cell microscopic imaging can be carried out with TPIPL from gold nanoplates and aggregated nanosphere. This method provided a platform for developing tools for biological and biomedical studies.
Keywords: Bio-imaging; Extinction spectra; Far-field; Glass substrates; Gold Nanoparticles; Gold nanoplates; Living cell imaging; Microscopic imaging; Nanoparticle aggregate; Nanoplates; SEM; Two photon; Two-photon laser scanning microscopies
Journal Title: Advanced Materials
Volume: 21
Issue 22
ISSN: 0935-9648
Publisher: Wiley-Blackwell  
Date Published: 2009-06-12
Start Page: 2309
End Page: 2313
DOI: 10.1002/adma.200802312
Open access: no