High-resolution quantitative visualization of glutamate and GABA receptors at central synapses Review Article


Author(s): Masugi-Tokita, Miwako; Shigemoto, Ryuichi
Title: High-resolution quantitative visualization of glutamate and GABA receptors at central synapses
Affiliation
Abstract: Glutamate and GABA are the main transmitters in the central nervous system and their effects are mediated by ionotropic and metabotropic receptors. Immunogold electron microscopy has revealed the quantitative localization of these receptors at 20-30 nm resolution. SDS-digested freeze-fracture replica labeling (SDS-FRL), a newly developed immunogold method, provides an accurate estimate of molecule numbers. Here, we summarize the recent advances in quantitative receptor localization, including use of SDS-FRL analyses to determine numbers of AMPA-type glutamate receptors in the cerebellum. The two-dimensional view and high sensitivity of SDS-FRL have revealed small, irregularly shaped AMPA receptor clusters within cerebellar synapses.
Keywords: Glutamate receptor; AMPA receptor; synapse receptor; 4 aminobutyric acid receptor; ionotropic receptor; presynaptic receptor
Publication Title: Current Opinion in Neurobiology
Volume: 17
Issue 3
ISBN: 0959-4388
Publisher: Elsevier  
Date Published: 2007-06-01
Start Page: 387
End Page: 393
DOI: 10.1016/j.conb.2007.04.012
Open access: no