Depression of GABAergic input to identified hippocampal neurons by group III metabotropic glutamate receptors in the rat Journal Article


Author(s): Kogo, Naoki; Dalezios, Yannis; Capogna, Marco; Ferraguti, Francesco; Shigemoto, Ryuichi; Somogyi, Péter
Article Title: Depression of GABAergic input to identified hippocampal neurons by group III metabotropic glutamate receptors in the rat
Affiliation
Abstract: The release of GABA in synapses is modulated by presynaptic metabotropic glutamate receptors (mGluRs). We tested whether GABA release to identified hippocampal neurons is influenced by group III mGluR activation using the agonist L-(+)-2-amino-4-phosphonobutyric acid (L-AP4) on inhibitory postsynaptic currents (IPSCs) evoked in CA1 interneurons and pyramidal cells. In interneurons, characterized with biocytin and immunolabelling for somatostatin, evoked IPSCs were depressed by 50 μM L-AP4 (activating mGluR4 and 8) to 68±6% of control, but they were rarely depressed in pyramidal cells (96±4% of control). At 300-500 μM concentration (activating mGluR4, 7 and 8), L-AP4 depressed IPSCs in both interneurons (to 70±6%) and pyramidal cells (to 67±4%). The change in trial-to-trial variability and in paired-pulse depression indicated a presynaptic action. In interneurons, the degree of IPSC depression was variable (to 9-87%), and a third of IPSCs were not affected by L-AP4. The L-AP4-evoked IPSC depression was blocked by LY341495. The depression of IPSCs was similar in O-LM cells and other interneurons. The lack of cell-type selectivity and the similar efficacy of different concentrations of L-AP4 suggest that several group III mGluRs are involved in the depression of IPSCs. Electron microscopic immunocytochemistry confirmed that mGluR4, mGluR7a and mGluR8a occur in the presynaptic active zone of GABAergic terminals on interneurons, but not on those innervating pyramidal cells. The high variability of L-AP4-evoked IPSC suppression is in line with the selective expression of presynaptic mGluRs by several distinct types of GABAergic neuron innervating each interneuron type.
Keywords: Hippocampus; inhibition; GABA; Presynaptic; IPSC
Journal Title: European Journal of Neuroscience
Volume: 19
Issue 10
ISSN: 1460-9568
Publisher: Wiley  
Date Published: 2004-05-01
Start Page: 2727
End Page: 2740
DOI: 10.1111/j.0953-816X.2004.03394.x
Open access: no