Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site Journal Article


Author(s): Boudin, Hélène; Doan, Andrew; Xia, Jun; Shigemoto, Ryuichi; Huganir, Richard L; Worley, Paul F; Craig, Ann M
Article Title: Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site
Affiliation
Abstract: Aggregation of neurotransmitter receptors at pre- and postsynaptic structures is crucial for efficient neuronal communication. In contrast to the wealth of information about postsynaptic specializations, little is known about the molecular organization of presynaptic membrane proteins. We show here that the metabotropic glutamate receptor mGluR7a, which localizes specifically to presynaptic active zones, interacts in vitro and in vivo with PICK1. Coexpression in heterologous systems induces coclustering dependent upon the extreme C terminus of mGluR7a and the PDZ domain of PICK1. mGluR7a and PICK1 localize to excitatory synapses in hippocampal neurons. Furthermore, whereas transfected mGluR7a clusters at presynaptic sites, mGluR7aΔ3 lacking the PICK1 binding site targets to axons but does not cluster. These results suggest that PICK1 is a component of the presynaptic machinery involved in mGlUR7a aggregation and in modulation of glutamate neurotransmission.
Keywords: membrane protein; Glutamic Acid; unclassified drug; neurotransmitter receptor; metabotropic receptor; protein interacting with protein kinase C
Journal Title: Neuron
Volume: 28
Issue 2
ISSN: 0896-6273
Publisher: Elsevier  
Date Published: 2000-11-01
Start Page: 485
End Page: 497
DOI: 10.1016/S0896-6273(00)00127-6
Open access: no