Smooth muscle cells are the site of neurokinin-1 receptor localization in the arterial supply of the rat sciatic nerve Journal Article


Author(s): Kummer, Wolfgang; Shigemoto, Ryuichi; Haberberger, Rainer V
Article Title: Smooth muscle cells are the site of neurokinin-1 receptor localization in the arterial supply of the rat sciatic nerve
Affiliation
Abstract: The occurrence and distribution of the preferred receptor for the neuropeptide, substance P (SP), the neurokinin-1 receptor (NK1R) was investigated in the vascular supply of the rat sciatic nerve. Messenger RNA for NK1R was demonstrated by RT-PCR in the epineurial layer where the majority of small arteries and arterioles feeding the endoneurial vasculature are located. Immunoreactivity to NK1R-protein was localized on the smooth muscle cells of these arterial vessels by means of immunofluorescence using a polyclonal NK1R antiserum. This muscular localization of NK1R explains the previously reported [Zochodne, D.W. and Ho, L.T., J. Physiol. 444 (1991) 615- 630] moderate vasoconstrictor rather than vasodilator effects of SP in this vascular bed.
Keywords: Immunohistochemistry; Substance P; Substance P receptor; Artery; Peripheral nerve; Tachykinin receptor; Vasa nervorum
Journal Title: Neuroscience Letters
Volume: 259
Issue 2
ISSN: 0304-3940
Publisher: Elsevier  
Date Published: 1999-01-08
Start Page: 119
End Page: 122
DOI: 10.1016/S0304-3940(98)00926-4
Open access: no