The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase Journal Article


Author(s): Tone, Yoshinori; Inoue, Hiroyasu; Hara, Shuntaro; Yokoyama, Chieko; Hatae, Toshihisa; Oida, Hiroji; Narumiya, Shuh; Shigemoto, Ryuichi; Yukawa, Susumu; Tanabe, Tadashi
Article Title: The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase
Affiliation
Abstract: The cloned cDNA for rat prostacyclin synthase was found to contain a 1503-bp open reading frame which encoded a 501-amino acid protein sharing 84% identity with the human enzyme. RNA blot analysis revealed that the rat prostacyclin synthase mRNA, as a single species of 2.1 kb, is expressed abundantly in the aorta and uterus. High levels of expression were also observed in the stomach, lung, heart, testis, liver, and skeletal muscle. Low but significant expression was also seen in the brain and kidney. Furthermore, the regional distribution and cellular localization of prostacyclin synthase mRNA were examined by in situ hybridization analysis of rat tissue sections. The definitive signals for the mRNA were localized in smooth muscle cells of the arteries, bronchi and uterus, and in the cells of the fibrous tunic surrounding the seminiferous tubules, which are characterized as smooth muscle cells. Besides smooth muscle cells, signal were also detected in the fibroblasts of the heart myocardium, lung parenchyma cells and kidney inner medulla tubules and interstitial cells.
Keywords: Distribution; Expression; mRNA; Prostacyclin synthase
Journal Title: European Journal of Cell Biology
Volume: 72
Issue 3
ISSN: 0070-2463
Publisher: Elsevier  
Date Published: 1997-03-01
Start Page: 268
End Page: 277
Open access: no