Pre- and postsynaptic localization of a metabotropic glutamate receptor, mGluR2, in the rat brain: An immunohistochemical study with a monoclonal antibody Journal Article


Author(s): Neki, Akio; Ohishi, Hitoshi; Kaneko, Takeshi; Shigemoto, Ryuichi; Nakanishi, Shigetada; Mizuno, Noboru
Article Title: Pre- and postsynaptic localization of a metabotropic glutamate receptor, mGluR2, in the rat brain: An immunohistochemical study with a monoclonal antibody
Affiliation
Abstract: A monoclonal antibody against a metabotropic glutamate receptor, mGluR2, was produced by using a glutathione S-transferase (GST) fusion protein containing an N-terminal sequence of rat mGluR2. Intense mGluR2-like immunoreactivity (mGluR2-LI) was seen mainly in neuropil of the cerebral cortical regions, hippocampus, olfactory bulb, some diencephalic nuclei, dorsal cochlear nucleus and cerebellar cortex. In the cerebellar cortex, mGluR2-LI was seen only in Golgi cells. In Ammon's hem, mGluR2-LI was marked in the stratum lucidum of CA3 and the stratum lacunosum-moleculare of CA1-CA3, but not detected in the stratum pyramidale. The results indicate that mGluR2 is located not only presynaptically but also postsynaptically.
Keywords: Immunohistochemistry; Cerebellum; Hippocampus; Metabotropic glutamate receptors; MGluR2; Monoclonal antibody; Trans-1-aminocyclopentane-1,3-dicarboxylate (tACPD)
Journal Title: Neuroscience Letters
Volume: 202
Issue 3
ISSN: 0304-3940
Publisher: Elsevier  
Date Published: 1996-01-05
Start Page: 197
End Page: 200
DOI: 10.1016/0304-3940(95)12248-6
Open access: no