Immunohistochemical localization of metabotropic glutamate receptors, mGluR2 and mGluR3, in rat cerebellar cortex Journal Article


Author(s): Ohishi, Hitoshi; Ogawa-Meguro, Reiko; Shigemoto, Ryuichi; Kaneko, Takeshi; Nakanishi, Shigetada; Mizuno, Noboru
Article Title: Immunohistochemical localization of metabotropic glutamate receptors, mGluR2 and mGluR3, in rat cerebellar cortex
Affiliation
Abstract: The distribution of the metabotropic glutamate receptors mGluR2 and mGluR3 was immunohistochemically examined in the rat cerebellar cortex at both light and electron microscope levels. An antibody was raised against a fusion protein containing a C-terminal portion of mGluR2. On immunoblot, the antibody reacted with both mGluR2 and mGluR3 in rat brain. mGluR2/3 immunoreactivity was expressed in cell bodies, dendrites, and axon terminals of Golgi cells, as well as in presumed glial processes. Golgi axon terminals with mGluR2/3 immunoreactivity were often encountered in the vicinity of glutamatergic mossy fiber terminals. The results suggest that transmitter glutamate may exert control influences upon Golgi cells not only through dendritic mGluR2/3, but also through axonal mGluR2/3.
Keywords: metabotropic receptor; Monoclonal antibody
Journal Title: Neuron
Volume: 13
Issue 1
ISSN: 0896-6273
Publisher: Elsevier  
Date Published: 1994-07-01
Start Page: 55
End Page: 66
DOI: 10.1016/0896-6273(94)90459-6
Open access: no