Antibodies inactivating mGluR1 metabotropic glutamate receptor block long-term depression in cultured Purkinje cells Journal Article


Author(s): Shigemoto, Ryuichi; Abe, Takaaki; Nomura, Sakashi; Nakanishi, Shigetada; Hirano, Tomoo
Article Title: Antibodies inactivating mGluR1 metabotropic glutamate receptor block long-term depression in cultured Purkinje cells
Affiliation
Abstract: Antibodies were raised against two distinct extracellular sequences of the rat mGluR1 metabotropic glutamate receptor expressed as bacterial fusion proteins. Both antibodies specifically reacted with mGluR1 in the rat cerebellum and inhibited the mGluR1 activity as assessed by the analysis of glutamate-stimulated inositol phosphate formation in CHO cells expressing mGluR1. Using these antibodies, we examined the role of mGluR1 in the induction of long-term depression in cultured Purkinje cells. In voltage- clamped Purkinje cells, current induced by iontophoretically applied glutamate was persistently depressed by depolarization of the Purkinje cells in conjunction with the glutamate application. The mGluR1 antibodies completely blocked the depression of glutamate-induced current. The results indicate that activation of mGluR1 is necessary for the induction of cerebellar long-term depression and that these mGluR1 antibodies can be used as selective antagonists.
Keywords: Glutamate receptor; metabotropic receptor; antibody; bacterial protein; hybrid protein
Journal Title: Neuron
Volume: 12
Issue 6
ISSN: 0896-6273
Publisher: Elsevier  
Date Published: 1994-06-01
Start Page: 1245
End Page: 1255
DOI: 10.1016/0896-6273(94)90441-3
Open access: no