Functional expression and tissue distribution of a novel receptor for vasoactive intestinal polypeptide Journal Article


Author(s): Ishihara, Takeshi; Shigemoto, Ryuichi; Mori, Kensaku; Takahashi, Kenji; Nagata, Shigekazu
Article Title: Functional expression and tissue distribution of a novel receptor for vasoactive intestinal polypeptide
Affiliation
Abstract: Vasoactive intestinal polypeptide (VIP), a 28 amino acid peptide hormone, plays many physiological roles in the peripheral and central nerve systems. A functional cDNA clone of the VIP receptor was isolated from a rat lung cDNA library by cross-hybridization with the secretin receptor cDNA. VIP bound the cloned VIP receptor expressed in mouse COP cells and stimulated adenylate cyclase through the cloned receptor. The rat VIP receptor consists of 459 amino acids with a calculated Mr of 52,054 and contains seven transmembrane segments. It is structurally related to the secretin, calcitonin, and parathyroid hormone receptors, suggesting that they constitute a new subfamily of the G5 protein - coupled receptors. VIP receptor mRNA was detected in various rat tissues including liver, lung, intestines, and brain. In situ hybridization revealed that VIP receptor mRNA is widely distributed in neuronal cells of the adult rat brain, with a relatively high expression in the cerebral cortex and hippocampus.
Keywords: Vasoactive intestinal polypeptide; cyclic AMP; complementary dna; messenger rna; adenylate cyclase; glucagon; secretin; vasoactive intestinal polypeptide receptor; amino acid; calcitonin receptor; parathyroid hormone receptor
Journal Title: Neuron
Volume: 8
Issue 4
ISSN: 0896-6273
Publisher: Elsevier  
Date Published: 1992-04-01
Start Page: 811
End Page: 819
DOI: 10.1016/0896-6273(92)90101-I
Open access: no