Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split Journal Article


Author(s): Sasai, Yoshiki; Kageyama, Ryoichiro; Tagawa, Yoshiaki; Shigemoto, Ryuichi; Nakanishi, Shigetada
Article Title: Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split
Affiliation
Abstract: We report the molecular characterization of two novel rat helix-loop-helix (HLH) proteins, designated HES-1 and HES-3, that show structural homology to the Drosophila hairy and Enhancer of split [E(spl)] proteins, both of which are required for normal neurogenesis. HES-1 mRNA, expressed in various tissues of both embryos and adults, is present at a high level in the epithelial cells, including the embryonal neuroepithelial cells, as well as in the mesoderm-derived tissues such as the embryonal muscle. In contrast, HES-3 mRNA is produced exclusively in cerebellar Purkinje cells. HES-1 represses transcription by binding to the N box, which is a recognition sequence of E(spl) proteins. Interestingly, neither HES-1 nor HES-3 alone interacts efficiently with the E box, but each protein decreases the transcription induced by E-box-binding HLH activators such as E47. Furthermore, HES-1 also inhibits the functions of MyoD and MASH1 and effectively diminishes the myogenic conversion of C3H10T1/2 cells induced by MyoD. These results suggest that HES-1 may play an important role in mammalian development by negatively acting on the two different sequences while HES-3 acts as a repressor in a specific type of neurons.
Keywords: Purkinje cell; Enhancer of split; Hairy; Helix-loop-helix proteins; Neuroepithelium; Repressor
Journal Title: Genes and Development
Volume: 6
Issue 12 B
ISSN: 1549-5477
Publisher: Cold Spring Harbor Laboratory Press  
Date Published: 1992-01-01
Start Page: 2620
End Page: 2634
DOI: 10.1101/gad.6.12b.2620
Open access: no