Metabolism of glutamate and ammonia in astrocyte an immunocytochemical study Journal Article


Author(s): Kaneko, Takeshi; Shigemoto, Ryuichi; Mizuno, Noboru
Article Title: Metabolism of glutamate and ammonia in astrocyte an immunocytochemical study
Affiliation
Abstract: Alpha-ketoglutamate (α-KG) reductive amination activity in rat brain was found to be mostly absorbed with an antibody against liver glutamate dehydrogenase. With this and anti-glutamine synthetase antibodies, α-KG reductive amination activity was immunocytochemically shown to coexist with glutamine synthetase activity in astrocytes. The results suggest that astrocytes de novo synthesize glutamate from α-KG and ammonia, and metabolize it to glutamine.
Keywords: Glutamate; glia; Glutaminase; α-Ketoglutarate; Ammonia; Astrocyte; Glutamate dehydrogenase; Glutamine synthetase
Journal Title: Brain Research
Volume: 457
Issue 1
ISSN: 0006-8993
Publisher: Elsevier  
Date Published: 1988-08-02
Start Page: 160
End Page: 164
DOI: 10.1016/0006-8993(88)90069-8
Open access: no