Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo Journal Article


Author(s): Grinevich, Valery V; Kolleker, Alexander; Eliava, Marina I; Takada, Naoki; Takuma, Hiroshi; Fukazawa, Yugo; Shigemoto, Ryuichi; Kuhl, Dietmar; Waters, Jack; Seeburg, Peter H; Osten, Pavel
Article Title: Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo
Affiliation
Abstract: The brain-specific immediate early gene Arc/Arg3.1 is induced in response to a variety of stimuli, including sensory and behavior-linked neural activity. Here we report the generation of transgenic mice, termed TgArc/Arg3.1-d4EGFP, expressing a 4-h half-life form of enhanced green fluorescent protein (d4EGFP) under the control of the Arc/Arg3.1 promoter. We show that d4EGFP-mediated fluorescence faithfully reports Arc/Arg3.1 induction in response to physiological, pathological and pharmacological stimuli, and that this fluorescence permits electrical recording from activated neurons in the live mouse. Moreover, the fluorescent Arc/Arg3.1 indicator revealed activity changes in circumscribed brain areas in distinct modes of stress and in a mouse model of Alzheimer's disease. These findings identify the TgArc/Arg3.1-d4EGFP mouse as a versatile tool to monitor Arc/Arg3.1 induction in neural circuits, both in vitro and in vivo.
Keywords: Synaptic plasticity; Arc; Arg3.1; Green fluorescent protein (GFP); Immediate early genes; Neural circuits; Stress; Transgenic mice
Journal Title: Journal of Neuroscience Methods
Volume: 184
Issue 1
ISSN: 0165-0270
Publisher: Elsevier  
Date Published: 2009-10-30
Start Page: 25
End Page: 36
DOI: 10.1016/j.jneumeth.2009.07.015
Open access: no