Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development Journal Article


Author(s): Cazzonelli, Christopher I; Vanstraelen, Marleen; Simon, Sibu; Yin, Kuide; Carron-Arthur, Ashley; Nisar, Nazia; Tarle, Gauri; Cuttriss, Abby J; Searle, Iain R; Benková, Eva; Mathesius, Ulrike; Masle, Josette; Friml, Jiří; Pogson, Barry J
Article Title: Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development
Affiliation IST Austria
Abstract: Plant-specific PIN-formed (PIN) efflux transporters for the plant hormone auxin are required for tissue-specific directional auxin transport and cellular auxin homeostasis. The Arabidopsis PIN protein family has been shown to play important roles in developmental processes such as embryogenesis, organogenesis, vascular tissue differentiation, root meristem patterning and tropic growth. Here we analyzed roles of the less characterised Arabidopsis PIN6 auxin transporter. PIN6 is auxin-inducible and is expressed during multiple auxin-regulated developmental processes. Loss of pin6 function interfered with primary root growth and lateral root development. Misexpression of PIN6 affected auxin transport and interfered with auxin homeostasis in other growth processes such as shoot apical dominance, lateral root primordia development, adventitious root formation, root hair outgrowth and root waving. These changes in auxin-regulated growth correlated with a reduction in total auxin transport as well as with an altered activity of DR5-GUS auxin response reporter. Overall, the data indicate that PIN6 regulates auxin homeostasis during plant development.
Journal Title: PLoS One
Volume: 8
Issue 7
ISSN: 1932-6203
Publisher: Public Library of Science  
Date Published: 2013-07-29
Start Page: Article number: e70069
Copyright Statement: CC BY
URL:
DOI: 10.1371/journal.pone.0070069
Notes: CIC and BJP were supported by the Australian Research Council (ARC) Centre of Excellence in Plant Energy Biology (CE0561495). UM was supported by an ARC Future Fellowship (FT100100669). IS was supported by an ARC QEII Fellowship (DP988846). KY was supported by the chinese government on a visiting fellowship. This work was supported by a postdoctoral fellowship of the Research Foundation-Flanders (MV), and the European Research Council with a Starting Independent Research Grant (ERC-2007-Stg-207362-HCPO ) (to EB and ERC-2011-StG-20101109-PSDP to JF). The funders had no role in study design, data collection and analysis,decision to publish, or preparation of the manuscript.
Open access: yes (OA journal)