Dissection of gene function at clonal level using mosaic analysis with double markers Review Article


Author(s): Hippenmeyer, Simon
Title: Dissection of gene function at clonal level using mosaic analysis with double markers
Affiliation IST Austria
Abstract: MADM (Mosaic Analysis with Double Markers) technology offers a genetic approach in mice to visualize and concomitantly manipulate genetically defined cells at clonal level and single cell resolution. MADM employs Cre recombinase/loxP-dependent interchromosomal mitotic recombination to reconstitute two split marker genes-green GFP and red tdTomato - and can label sparse clones of homozygous mutant cells in one color and wild-type cells in the other color in an otherwise unlabeled background. At present, major MADM applications include lineage tracing, single cell labeling, conditional knockouts in small populations of cells and induction of uniparental chromosome disomy to assess effects of genomic imprinting. MADM can be applied universally in the mouse with the sole limitation being the specificity of the promoter controlling Cre recombinase expression. Here I review recent developments and extensions of the MADM technique and give an overview of the major discoveries and progresses enabled by the implementation of the novel genetic MADM tools.
Keywords: Neural development; clonal analysis; genetic mosaic; genomic imprinting; lineage tracing; MADM
Publication Title: Frontiers in Biology
Volume: 8
Issue 6
ISBN: 1674-7992
Publisher: Springer  
Date Published: 2013-12-01
Start Page: 557
End Page: 568
DOI: 10.1007/s11515-013-1279-6
Notes: I thank L. Luo and H. Zong for continued discussions about MADM and G. Arque for helpful comments on the manuscript. This work was supported by IST Austria institutional funds.
Open access: no