A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation Journal Article


Author(s): Dueck, Anne; Eichner, Alexander; Sixt, Michael; Meister, Gunter
Article Title: A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation
Affiliation IST Austria
Abstract: MicroRNAs (miRNAs) are small RNAs that play important regulatory roles in many cellular pathways. MiRNAs associate with members of the Argonaute protein family and bind to partially complementary sequences on mRNAs and induce translational repression or mRNA decay. Using deep sequencing and Northern blotting, we characterized miRNA expression in wild type and miR-155-deficient dendritic cells (DCs) and macrophages. Analysis of different stimuli (LPS, LDL, eLDL, oxLDL) reveals a direct influence of miR-155 on the expression levels of other miRNAs. For example, miR-455 is negatively regulated in miR-155-deficient cells possibly due to inhibition of the transcription factor C/EBPbeta by miR-155. Based on our comprehensive data sets, we propose a model of hierarchical miRNA expression dominated by miR-155 in DCs and macrophages.
Keywords: dendritic cell; MicroRNA; MiR-155; MiR-455; Regulation of gene expression; Macrophage
Journal Title: FEBS Letters
Volume: 588
Issue 4
ISSN: 0014-5793
Publisher: Elsevier  
Date Published: 2014-02-14
Start Page: 632
End Page: 640
DOI: 10.1016/j.febslet.2014.01.009
Notes: We thank S. Ammon and C. Friederich for technical assistance and Norbert Eichner for help with data processing. LDL, eLDL and oxLDL were kindly provided by Prof. G. Schmitz (Regensburg). Our research is supported by Grants from the Deutsche Forschungsgemeinschaft (SFB 960 projects B3 and B4 to G.M. and FOR855 to G.M.), the (ERC Grant 242792 ‘sRNAs’ to G.M.), the Bavarian Genome Research Network (BayGene to G.M.), the Bavarian Systems-Biology Network (BioSysNet to G.M.) and the European Union (Grant 201102 ‘ONCOMIRs’ to G.M.).
Open access: no