Loose coupling between Ca^2+ channels and release sensors at a plastic hippocampal synapse Journal Article

Author(s): Vyleta, Nicholas P; Jonas, Peter
Article Title: Loose coupling between Ca^2+ channels and release sensors at a plastic hippocampal synapse
Affiliation IST Austria
Abstract: The distance between Ca^2+ channels and release sensors determines the speed and efficacy of synaptic transmission. Tight "nanodomain" channel-sensor coupling initiates transmitter release at synapses in the mature brain, whereas loose "microdomain" coupling appears restricted to early developmental stages. To probe the coupling configuration at a plastic synapse in the mature central nervous system, we performed paired recordings between mossy fiber terminals and CA3 pyramidal neurons in rat hippocampus. Millimolar concentrations of both the fast Ca^2+ chelator BAPTA [1,2-bis(2-aminophenoxy)ethane- N,N, N′,N′-tetraacetic acid] and the slow chelator EGTA efficiently suppressed transmitter release, indicating loose coupling between Ca^2+ channels and release sensors. Loose coupling enabled the control of initial release probability by fast endogenous Ca^2+ buffers and the generation of facilitation by buffer saturation. Thus, loose coupling provides the molecular framework for presynaptic plasticity.
Keywords: Synaptic Transmission; Hippocampus; calcium channel; drug derivative; 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; chelating agent; egtazic acid; ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid; hippocampal CA3 region; hippocampal mossy fiber; metabolism; nerve cell plasticity
Journal Title: Science
Volume: 343
Issue 6171
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science  
Date Published: 2014-02-01
Start Page: 665
End Page: 670
DOI: 10.1126/science.1244811
Notes: Supported by the Fond zur Förderung der Wissenschaftlichen Forschung (P 24909-B24) and the European Union (European Research Council Advanced Grant 268548 to P.J.).
Open access: yes (repository)