Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences Journal Article


Author(s): Cires, Eduardo R; Baltisberger, Matthias; Cuesta Moliner, Candela; Vargas, Pablo; Prieto, José A
Article Title: Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences
Affiliation IST Austria
Abstract: The Balkan Peninsula, characterized by high rates of endemism, is recognised as one of the most diverse and species-rich areas of Europe. However, little is known about the origin of Balkan endemics. The present study addresses the phylogenetic position of the Balkan endemic Ranunculus wettsteinii, as well as its taxonomic status and relationship with the widespread R. parnassiifolius, based on nuclear DNA (internal transcribed spacer, ITS) and plastid regions (rpl32-trnL, rps16-trnQ, trnK-matK and ycf6-psbM). Maximum parsimony and Bayesian inference analyses revealed a well-supported clade formed by accessions of R. wettsteinii. Furthermore, our phylogenetic and network analyses supported previous hypotheses of a likely allopolyploid origin for R. wettsteinii between R. montenegrinus and R. parnassiifolius, with the latter as the maternal parent.
Keywords: Balkan Peninsula; Internal transcribed spacer; Plastid DNA; Ranunculus; Species trees; Systematic
Journal Title: Organisms Diversity and Evolution
Volume: 14
Issue 1
ISSN: 1439-6092
Publisher: Springer  
Date Published: 2014-03-01
Start Page: 1
End Page: 10
Sponsor: Part of this work was conducted during a short stay of E. Cires at Real Jardín Botánico de Madrid (CSIC) funded partially by the University of Oviedo (V20090232). This research was funded by the Spanish Ministry of Science (CGL2006-11743).
DOI: 10.1007/s13127-013-0150-6
Notes: The authors thank Emilio Cano for laboratory assistance and Bernardo García for helping with the sampling. We would like to thank three anonymous reviewers for useful comments that improved the manuscript.
Open access: no