Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis Journal Article


Author(s): Mazur, Ewa; Kurczyñska, Ewa U.; Friml, Jiřĺ
Article Title: Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis
Affiliation IST Austria
Abstract: Development of cambium and its activity is important for our knowledge of the mechanism of secondary growth. Arabidopsis thaliana emerges as a good model plant for such a kind of study. Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC). These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions. Distribution of auxin was observed to be higher in differentiating into cambium parenchyma cells compared to cells within the pith and cortex. Expression of PIN1 in IPC was always preceded by auxin accumulation. Basal localization of PIN1 was already established in the cells prior to their periclinal division. These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles. The final consequence of which was the closure of the cambial ring within the stem. Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs). In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis.
Keywords: Auxin; Arabidopsis thaliana; Cambium; Inflorescence stem; PIN1 protein; Secondary growth
Journal Title: Protoplasma
Volume: 251
Issue 5
ISSN: 1615-6102
Publisher: Springer  
Date Published: 2014-02-14
Start Page: 1125
End Page: 1139
DOI: 10.1007/s00709-014-0620-5
Notes: We express our gratitude to Professor Paul Knox for the anti-extensin LM1 antibody probe for HRGPs detection and Dr. Timothy C. Baldwin (University of Wolverhampton) for his assistance in the preparation of the English version of this manuscript.
Open access: no