Spatial regulators for bacterial cell division self-organize into surface waves in vitro Journal Article


Author(s): Loose, Martin; Fischer-Friedrich, Elisabeth; Ries, Jonas ; Kruse, Karsten; Schwille, Petra
Article Title: Spatial regulators for bacterial cell division self-organize into surface waves in vitro
Affiliation
Abstract: In the bacterium Escherichia coli, the Min proteins oscillate between the cell poles to select the cell center as division site. This dynamic pattern has been proposed to arise by self-organization of these proteins, and several models have suggested a reaction-diffusion type mechanism. Here, we found that the Min proteins spontaneously formed planar surface waves on a flat membrane in vitro. The formation and maintenance of these patterns, which extended for hundreds of micrometers, required adenosine 5′-triphosphate (ATP), and they persisted for hours. We present a reaction-diffusion model of the MinD and MinE dynamics that accounts for our experimental observations and also captures the in vivo oscillations.
Keywords: bacterial protein; adenosine triphosphatase; MinD protein; protein MinE; Bacteria (microorganisms); Escherichia coli adenosine triphosphate; MinC protein; cell cycle protein; cytoskeleton protein; Escherichia coli protein; FtsZ protein, Bacteria; MinD protein, E coli; MinE protein, E coli
Journal Title: Science
Volume: 320
Issue 5877
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science  
Date Published: 2008-05-09
Start Page: 789
End Page: 792
Sponsor: This work was supported by the Max-Planck-Society (M.L., P.S., E.F.).
DOI: 10.1126/science.1154413
Open access: no
IST Austria Authors
  1. Martin Loose
    13 Loose