Respiratory burst inhibition in human neutrophils by ultra-low doses of [D-Ala2] methionine enkephalinamide Journal Article


Author(s): Zaǐtsev, Sergei V; Sazanov, Leonid A; Koshkin, Aleksei A; Sud'Ina, Galina F; Varfolomeev, Sergei D
Article Title: Respiratory burst inhibition in human neutrophils by ultra-low doses of [D-Ala2] methionine enkephalinamide
Alternate Title: FEBS Letters
Affiliation
Abstract: An ultra-low dose (10-14 M) of opioid peptide [D-Ala2]methionine enkephalinamide (DAMEA) is found to exert an inhibitory effect on the production of reactive oxygen species (respiratory burst) in human neutrophils. The validity of this phenomenon has been verified in a series of studies that comprised 30 experiments. The inhibition has proved to be statistically significant (P<0.001). The dose-response dependence of the effect (10-15-10-9 M) followed a characteristic biphasic pattern (with the maximum effect at ultra-low doses). An opioid antagonist, naloxone partially blocks the inhibitory effect, which indicates that the DAMEA action is at least partially mediated by opioid receptors.
Keywords: Respiratory burst; Ultra-low dose; Human neutrophil; [D-Ala2]Met-enkephalinamide
Journal Title: FEBS Letters
Volume: 291
Issue 1
ISSN: 0014-5793
Publisher: Elsevier  
Date Published: 1991-10-07
Start Page: 84
End Page: 86
DOI: 10.1016/0014-5793(91)81109-L
Open access: no