Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates Journal Article

Author(s): Hatakeyama, Jun; Wakamatsu, Yoshio; Nagafuchi, Akira; Kageyama, Ryoichiro; Shigemoto, Ryuichi; Shimamura, Kenji
Article Title: Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates
Affiliation IST Austria
Abstract: The development of the vertebrate brain requires an exquisite balance between proliferation and differentiation of neural progenitors. Notch signaling plays a pivotal role in regulating this balance, yet the interaction between signaling and receiving cells remains poorly understood. We have found that numerous nascent neurons and/or intermediate neurogenic progenitors expressing the ligand of Notch retain apical endfeet transiently at the ventricular lumen that form adherens junctions (AJs) with the endfeet of progenitors. Forced detachment of the apical endfeet of those differentiating cells by disrupting AJs resulted in precocious neurogenesis that was preceded by the downregulation of Notch signaling. Both Notch1 and its ligand Dll1 are distributed around AJs in the apical endfeet, and these proteins physically interact with ZO-1, a constituent of the AJ. Furthermore, live imaging of a fluorescently tagged Notch1 demonstrated its trafficking from the apical endfoot to the nucleus upon cleavage. Our results identified the apical endfoot as the central site of active Notch signaling to securely prohibit inappropriate differentiation of neural progenitors.
Keywords: Cadherin; Neurogenesis; mouse; Adherens junction; Apical endfoot; Chick; Neural stem/progenitor cell; Notch signaling
Journal Title: Development
Volume: 141
Issue 8
ISSN: 1477-9129
Publisher: Company of Biologists  
Date Published: 2014-04-01
Start Page: 1671
End Page: 1682
Sponsor: This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan; from RIKEN CDB (to K.S.); from the Joint Usage/Research Center of Institute for Virus Research at Kyoto University (to J.H.); from t
DOI: 10.1242/dev.102988
Notes: We thank T. Muroi-Higuchi, T. Magarikaji and J. Kusuura for excellent technical assistance, and L. Kumar Parajuli, K. Furuta and M. Fujioka for assistance with the EM studies. We also thank Drs K. Araki, S. Chiba, A. Gossler, F. Guillemot, R. Kopan, M. Morimoto, H. Nakamura, Y. Saga, H. Sasaki, M. Takeichi, T. Tomita and K. Yamagata for providing mice or reagents.
Open access: no