Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis Journal Article


Author(s): Tejos, Ricardo I; Sauer, Michael; Vanneste, Steffen; Palacios-Gomez, Miriam; Li, Hongjiang; Heilmann, Mareike; Van Wijk, Ringo; Vermeer, Joop E; Heilmann, Ingo; Munnik, Teun; Friml, Jiří
Article Title: Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis
Affiliation IST Austria
Abstract: Cell polarity manifested by asymmetric distribution of cargoes, such as receptors and transporters, within the plasma membrane (PM) is crucial for essential functions in multicellular organisms. In plants, cell polarity (re)establishment is intimately linked to patterning processes. Despite the importance of cell polarity, its underlying mechanisms are still largely unknown, including the definition and distinctiveness of the polar domains within the PM. Here, we show in Arabidopsis thaliana that the signaling membrane components, the phosphoinositides phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 4, 5-bisphosphate [PtdIns(4, 5)P2] as well as PtdIns4P 5-kinases mediating their interconversion, are specifically enriched at apical and basal polar plasma membrane domains. The PtdIns4P 5-kinases PIP5K1 and PIP5K2 are redundantly required for polar localization of specifically apical and basal cargoes, such as PIN-FORMED transporters for the plant hormone auxin. As a consequence of the polarity defects, instructive auxin gradients as well as embryonic and postembryonic patterning are severely compromised. Furthermore, auxin itself regulates PIP5K transcription and PtdIns4P and PtdIns(4, 5)P2 levels, in particular their association with polar PM domains. Our results provide insight into the polar domain-delineating mechanisms in plant cells that depend on apical and basal distribution of membrane lipids and are essential for embryonic and postembryonic patterning.
Journal Title: Plant Cell
Volume: 26
Issue 5
ISSN: 1040-4651
Publisher: American Society of Plant Biologists  
Date Published: 2014-05-01
Start Page: 2114
End Page: 2128
Sponsor: This work was supported by grants from the Odysseus program of the Research Foundation-Flanders (to J.F.) and by ERC starting independent research Grant ERC-282300-PSDP (to J.F.). M.S. received funding from HFSPO, Marie Curie IEF, and European Molecular B
URL:
DOI: 10.1105/tpc.114.126185
Notes: We thank José Maria Carvajal for helpful discussions, Martine De Cock and Cecilia Rodriguez-Furlan for help during the preparation of the article, the ABRC for seed stocks, Agrisera for the kind gift of antibody samples, and Yvon Jaillais for sharing published material.
Open access: yes (repository)