Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules Journal Article


Author(s): Chen, Xu; Grandont, Laurie; Li, Hongjiang; Hauschild, Robert; Paque, Sébastien; Abuzeineh, Anas; Rakusová, Hana; Benková, Eva; Perrot-Rechenmann, Catherine; Friml, Jiří
Article Title: Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules
Affiliation IST Austria
Abstract: The prominent and evolutionarily ancient role of the plant hormone auxin is the regulation of cell expansion. Cell expansion requires ordered arrangement of the cytoskeleton but molecular mechanisms underlying its regulation by signalling molecules including auxin are unknown. Here we show in the model plant Arabidopsis thaliana that in elongating cells exogenous application of auxin or redistribution of endogenous auxin induces very rapid microtubule re-orientation from transverse to longitudinal, coherent with the inhibition of cell expansion. This fast auxin effect requires auxin binding protein 1 (ABP1) and involves a contribution of downstream signalling components such as ROP6 GTPase, ROP-interactive protein RIC1 and the microtubule-severing protein katanin. These components are required for rapid auxin-and ABP1-mediated re-orientation of microtubules to regulate cell elongation in roots and dark-grown hypocotyls as well as asymmetric growth during gravitropic responses.
Keywords: Auxin; Indoleacetic acid; green fluorescent protein; unclassified drug; vegetable protein; auxin binding protein 1; binding protein; microtubule associated protein 4
Journal Title: Nature
Volume: 516
Issue 729
ISSN: 0028-0836
Publisher: Nature Publishing Group  
Date Published: 2014-12-04
Start Page: 90
End Page: 93
URL:
DOI: 10.1038/nature13889
Notes: We thank R. Dixit for performing complementary experiments, D. W. Ehrhardt and T. Hashimoto for providing the seeds of TUB6–RFP and EB1b–GFP respectively, E. Zazimalova, J. Petrasek and M. Fendrych for discussing the manuscript and J. Leung for text optimization. This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP, to J.F.), ANR blanc AuxiWall project (ANR-11-BSV5-0007, to C.P.-R. and L.G.) and the Agency for Innovation by Science and Technology (IWT) (to H.R.). This work benefited from the facilities and expertise of the Imagif Cell Biology platform (http://www.imagif.cnrs.fr), which is supported by the Conseil Général de l’Essonne.
Open access: yes (repository)