Low-dose sevoflurane promoteshippocampal neurogenesis and facilitates the development of dentate gyrus-dependent learning in neonatal rats Journal Article


Author(s): Chen, Chong; Wang, Chao; Zhao, Xuan; Zhou, Tao; Xu, Dao-Jie; Wang, Zhi-Ru; Wang, Ying-Wei
Article Title: Low-dose sevoflurane promoteshippocampal neurogenesis and facilitates the development of dentate gyrus-dependent learning in neonatal rats
Affiliation IST Austria
Abstract: Huge body of evidences demonstrated that volatile anesthetics affect the hippocampal neurogenesis and neurocognitive functions, and most of them showed impairment at anesthetic dose. Here, we investigated the effect of low dose (1.8%) sevoflurane on hippocampal neurogenesis and dentate gyrus-dependent learning. Neonatal rats at postnatal day 4 to 6 (P4-6) were treated with 1.8% sevoflurane for 6 hours. Neurogenesis was quantified by bromodeoxyuridine labeling and electrophysiology recording. Four and seven weeks after treatment, the Morris water maze and contextual-fear discrimination learning tests were performed to determine the influence on spatial learning and pattern separation. A 6-hour treatment with 1.8% sevoflurane promoted hippocampal neurogenesis and increased the survival of newborn cells and the proportion of immature granular cells in the dentate gyrus of neonatal rats. Sevoflurane-treated rats performed better during the training days of the Morris water maze test and in contextual-fear discrimination learning test. These results suggest that a subanesthetic dose of sevoflurane promotes hippocampal neurogenesis in neonatal rats and facilitates their performance in dentate gyrus-dependent learning tasks.
Keywords: Neurogenesis; dentate gyrus; neonate; pattern separation; sevoflurane; spatial learning
Journal Title: ASN Neuro
Volume: 7
Issue 2
ISSN: 1759-0914
Publisher: SAGE  
Date Published: 2015-04-13
Start Page: Article number: 1759091415575845
Copyright Statement: CC BY 3.0
Sponsor: This work was supported by the grant from The National Natural Science Funds of China (81171169, 81200942 & 81300962), The Shanghai Health Commission Grant (XBR2011023), and The Shanghai Municipal Science and Technology Commission (14XD1402900).
URL:
DOI: 10.1177/1759091415575845
Notes: The authors are grateful to Dr. Christopher Lingle (Professor of Anesthesiology at Washington University School of Medicine) for his constructive suggestions.
Open access: yes (OA journal)