Transcriptional code and disease map for adult retinal cell types Journal Article


Author(s): Siegert, Sandra; Cabuy, Erik; Scherf, Brigitte G; Kohler, Hubertus; Panda, Satchidananda; Le, Yunzheng; Fehling, Hans J; Gaidatzis, Dimos; Stadler, Michael B E; Roska, Botond M
Article Title: Transcriptional code and disease map for adult retinal cell types
Affiliation
Abstract: Brain circuits are assembled from a large variety of morphologically and functionally diverse cell types. It is not known how the intermingled cell types of an individual adult brain region differ in their expressed genomes. Here we describe an atlas of cell type transcriptomes in one brain region, the mouse retina. We found that each adult cell type expressed a specific set of genes, including a unique set of transcription factors, forming a 'barcode' for cell identity. Cell type transcriptomes carried enough information to categorize cells into morphological classes and types. Several genes that were specifically expressed in particular retinal circuit elements, such as inhibitory neuron types, are associated with eye diseases. The resource described here allows gene expression to be compared across adult retinal cell types, experimenting with specific transcription factors to differentiate stem or somatic cells to retinal cell types, and predicting cellular targets of newly discovered disease-associated genes.
Keywords: Gene Expression; DNA barcoding; Retina; animal tissue; nerve cell; stem cell; transcription factor; transcriptome; brain region; disease association; eye disease; gene mapping; nucleotide sequence; retina cell; somatic cell
Journal Title: Nature Neuroscience
Volume: 15
Issue 3
ISSN: 1546-1726
Publisher: Nature Publishing Group  
Date Published: 2012-03-01
Start Page: 487
End Page: 495
DOI: 10.1038/nn.3032
Notes: The study was supported by Friedrich Miescher Institute funds, Alcon award, a National Center of Competence in Research Genetics grant, a European Research Council grant, a Swiss-Hungarian grant, and RETICIRC, TREATRUSH, SEEBETTER and OPTONEURO grants from the European Union to B.R.
Open access: no