Local retinal circuits of melanopsin-containing ganglion cells identified by transsynaptic viral tracing Journal Article


Author(s): Viney, Tim J; Bálint, Kamill; Hillier, Dániel; Siegert, Sandra; Boldogköi, Zsolt S; Enquist, Lynn W; Meister, Markus; Cepko, Constance L L; Roska, Botond M
Article Title: Local retinal circuits of melanopsin-containing ganglion cells identified by transsynaptic viral tracing
Affiliation
Abstract: Intrinsically photosensitive melanopsin-containing retinal ganglion cells (ipRGCs) control important physiological processes, including the circadian rhythm, the pupillary reflex, and the suppression of locomotor behavior (reviewed in [1]). ipRGCs are also activated by classical photoreceptors, the rods and cones, through local retinal circuits [2, 3]. ipRGCs can be transsynaptically labeled through the pupillary-reflex circuit with the derivatives of the Bartha strain of the alphaherpesvirus pseudorabies virus(PRV) [4, 5] that express GFP [6-12]. Bartha-strain derivatives spread only in the retrograde direction [13]. There is evidence that infected cells function normally for a while during GFP expression [7]. Here we combine transsynaptic PRV labeling, two-photon laser microscopy, and electrophysiological techniques to trace the local circuit of different ipRGC subtypes in the mouse retina and record light-evoked activity from the transsynaptically labeled ganglion cells. First, we show that ipRGCs are connected by monostratified amacrine cells that provide strong inhibition from classical-photoreceptor-driven circuits. Second, we show evidence that dopaminergic interplexiform cells are synaptically connected to ipRGCs. The latter finding provides a circuitry link between light-dark adaptation and ipRGC function.
Keywords: SYSNEURO
Journal Title: Current Biology
Volume: 17
Issue 11
ISSN: 0960-9822
Publisher: Cell Press  
Date Published: 2007-06-05
Start Page: 981
End Page: 988
DOI: 10.1016/j.cub.2007.04.058
Notes: This study was supported by Office of Naval Research Multidisciplinary University Research Initiative [ONR MURI] and Naval International Cooperative Opportunities in Science and Technology Program [NICOP] grants, a Marie Curie Excellence Grant, a Human Frontier Science Program [HFSP] Young Investigator grant, and Friedrich Miescher Institute funds to B.R.
Open access: no