Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors Journal Article


Author(s): Siegert, Sandra; Thaler, Sonja; Wagner, Ralf; Schnierle, Barbara S
Article Title: Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors
Affiliation
Abstract: Background: Murine leukemia virus (MLV) vector particles can be pseudotyped with a truncated variant of the human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) and selectively target gene transfer to human cells expressing both CD4 and an appropriate co-receptor. Vector transduction mimics the HIV-1 entry process and is therefore a safe tool to study HIV-1 entry. Results: Using FLY cells, which express the MLV gag and pol genes, we generated stable producer cell lines that express the HIV-1 envelope gene and a retroviral vector genome encoding the green fluorescent protein (GFP). The BH10 or 89.6 P HIV-1 Env was expressed from a bicistronic vector which allowed the rapid selection of stable cell lines. A codon-usage-optimized synthetic env gene permitted high, Rev-independent Env expression. Vectors generated by these producer cells displayed different sensitivity to entry inhibitors. Conclusion: These data illustrate that MLV/HIV-1 vectors are a valuable screening system for entry inhibitors or neutralizing antisera generated by vaccines.
Keywords: green fluorescent protein; codon; Human immunodeficiency virus vaccine; neutralizing antibody; virus envelope protein; genetic code; Human immunodeficiency virus 1; Murine leukemia virus; retrovirus vector; screening test; structural gene; virus genome; virus inhibition
Journal Title: AIDS Research and Therapy
Volume: 2
Issue 1
ISSN: 17426405
Publisher: BioMed Central  
Date Published: 2005-09-12
Start Page: Article number 7
DOI: 10.1186/1742-6405-2-7
Open access: yes (OA journal)