Light-assisted small-molecule screening against protein kinases Journal Article

Author(s): Ingles-Prieto, Álvaro; Reichhart, Eva; Muellner, Markus K; Nowak, Matthias; Nijman, Sebastian M; Grusch, Michael; Janovjak, Harald
Article Title: Light-assisted small-molecule screening against protein kinases
Affiliation IST Austria
Abstract: High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes.
Journal Title: Nature Chemical Biology
Volume: 11
ISSN: 1552-4469
Publisher: Nature Publishing Group  
Date Published: 2015-12-01
Start Page: 952
End Page: 954
DOI: 10.1038/nchembio.1933
Notes: This work was supported by grants from the European Union Seventh Framework Programme (CIG-303564 to H.J. and ERC-StG-311166 to S.M.B.N.), the Human Frontier Science Program (RGY0084_2012 to H.J.) and the Herzfelder Foundation (to M.G.). A.I.-P. was supported by a Ramon Areces fellowship, and E.R. by the graduate program MolecularDrugTargets (Austrian Science Fund (FWF): W 1232) and a FemTech fellowship (3580812 Austrian Research Promotion Agency).
Open access: yes (repository)